Histological Techniques
InhaltsangabeForeword.- Note concerning textual conventions.- Histological research and diagnostic histology.- Automatic, easy, difficult and capricious technique.- Choice of a technique.- Conclusion of technique.- one General Principles of Histological Technique.- 1. - Vital examination.- Removal of pieces.- Making preparations.- Examination under the light microscope.- Examination under the dark-field microscope.- Examination under the phase-contrast and interference microscope.- 2. - Vital Staining.- Definition.- Objects, advantages and disadvantages.- Classification of vital stains.- Theories of vital staining.- General rules for applying vital stains.- 3. - Fixation.- Classification and mode of action of fixatives.- Fixation by physical agents.- Fixation by chemical agents.- General remarks on fixative mixtures.- Fixative mixtures containing an "indifferent" salt.- Mixtures containing several fixing agents.- Period of fixation.- Temperature of fixation.- Modes of fixation.- Fixation with vapours.- Fixation in liquids.- Choice of fixative.- 4. - Embedding and preparatory operations.- Termination of fixation.- Embedding in paraffin.- Properties of the embedding medium.- Procedure of paraffin embedding.- Choice of method for paraffin embedding and summary of operating procedure.- Embedding in nitrocellulose (celloidin, collodion).- Properties of the embedding mass.- Procedure of embedding.- Embedding in gelatin.- Embedding in gelatin after Apathy.- Embedding in gelatin after Heringa and Ten Berge.- Embedding in gelatin after Baker.- Embedding in gelatin after Pearse.- Other methods of embedding.- The solid polyethylene-glycols.- Steedman's ester-wax.- Double embedding.- Embedding in agar-paraffin.- Embedding in celloidin-paraffin: the method of Apathy-the method of Pfuhl.- 5. - Cutting and sticking sections.- The razor.- The razor-holder.- The object-holder.- The mechanism of advance.- Choice of a microtome.- Maintenance of the microtome.- Cutting and handling paraffin sections.- Preparing the block.- Cutting sections.- Handling and spreading sections.- Cutting sections in paraffin-agar.- Cutting and handling celloidin and celloidin-paraffin sections.- Preparation of celloidin blocks to be cut under alcohol.- Preparation of celloiding blocks to be cut under terpineol.- Preparation of celloidin-paraffin blocks.- Cutting sections.- Sticking sections.- Cutting and handling gelatin sections.- Cutting and handling frozen sections.- Preparing blocks.- Cutting sections.- Handling and sticking sections.- 6. - Staining and mounting microscopical preparations.- Definition and nomenclature.- General theory of staining and the classification of stains.- Theories of histological staining.- The chemical theory.- The concept of staining by inhibition and by precipitation.- Adsorption as a factor in the binding of stains.- Electrostatic adsorption as a mechanism of histological staining.- Theory of indirect staining.- The density of structures as a factor in histological staining.- The texture of tissue constituents and their penetration by stains.- General remarks on the practice of staining.- Choice of stains.- Preparation of solutions.- Glassware.- Deparaffining sections.- Collodioning sections.- Dissolving crystals of mercury.- Summary of operations preceding the staining of sections.- Mounting media miscible in water: Apathy's syrup; The glycerine gum; Laevulose syrup; Gelatinized glycerine.- Mounting media miscible with benzenic hydrocarbons.- Summary of different mounting procedures.- Luting the preparations.- Conservation of microscopical preparations.- 7. - The examination of histological preparations.- The graphical representation of histological preparations.- The reconstruction of histological preparations.- Graphical reconstruction.- Plastic reconstruction.- The quantitative study of histological preparations.- The causes of error in measuring under the microscope.- Measuring lengths in the plane of the stage.- Measuring lengths along the o
€106.99
(inklusive MwSt.)
Verfügbarkeit: Besorgungstitel, Festbezug
Zusatztext
'Eyw rpwvry ~OiWTO~ EV Tli E(}'rllup JOHN, J, 23 A SURVEY OF THE PRINCIPAL WORKS on histological technique that have th appeared since the end of the 19 century shows such a diversity of concept and directive ideas, in spite of the fundamental unity of subject, that the choice of the underlying orientation was perhaps the most difficult problem facing me when MM. Masson & Cie, requested me to write this book. A classification of these works based on their particular orientation and carrying the excess inherent in all that is schematic would lead to the definition of three types of books. Some are handbooks or treatises on "microscopy". They are tacitly limited to the biological applications of the microscope, all of which are reviewed, and include in the same volume the techniques of histology proper along with those of bacteriology, botany and embryology. Such a work, on the scale of an encyclopaedic treatise contributed by specialists from a number of discip lines and meant to be consulted as a dictionary, may have a certain value if it is really exhaustive and periodically revised. But it is no longer possible on the scale of a handbook. A single person, however gifted, cannot have acquired sufficient mastery over all the techniques of the above disciplines to be able to speak from personal experience.
Weitere Details
Erschienen: 07.02.2012
Umfang: xxiv, 1108 S., 35 Tab.
Sprache: ENG
Einband: KT
ISBN/EAN: 9781461596400
Umbreit-Nr.: 5652384
