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Difference Gel Electrophoresis (DIGE)
Methods and Protocols, Methods in Molecular Biology 854
ISBN/EAN: 9781617795725
Umbreit-Nr.: 1282923
Sprache:
Englisch
Umfang: xiii, 401 S.
Format in cm:
Einband:
gebundenes Buch
Erschienen am 07.02.2012
Auflage: 1/2012
- Zusatztext
- InhaltsangabePart I. Fundamentals 1. DIGE: Past and Future Jonathan S. Minden 2. The Basics of 2D DIGE Phil Beckett 3. Multifluorescence 2D Gel Imaging and Image Analysis Ingo Vormbrock, Sonja Hartwig, and Stefan Lehr 4. Assessing Signal-to-Noise in Quantitative Proteomics: Multivariate Statistical Analysis in DIGE Experiments David B. Friedman 5. Analysis of Proteins Using DIGE and MALDI Mass Spectrometry Witold M. Winnik, Robert M. DeKroon, Joseph S. Y. Jeong, Mihaela Mocanu, Jennifer B. Robinette, Christina Osorio, Nedyalka N. Dicheva, Eric Hamlett, and Oscar Alzate 6. Synthesis and Validation of Cyanin-Based Dyes for DIGE Michael E. Jung, Wan-Joong Kim, Nuraly K. Avliyakulov, Merve Oztug, and Michael J. Haykinson Part II. Methods 7. 2D DIGE Saturation Labeling for Minute Sample Amounts Georg J. Arnold and Thomas Fröhlich 8. Proteomic Analysis of Redox-Dependent Changes Using Cysteine-Labeling 2D DIGE Hong-Lin Chan, John Sinclair, and John F. Timms 9. Analysis of Protein Post-Translational Modifications Using DIGE-Based Proteomics Robert M. DeKroon, Jennifer B. Robinette, Cristina Osorio, Joseph S. Y. Jeong, Eric Hamlett, Mihaela Mocanu, and Oscar Alzate 10. Comparative Analyses of Protein Complexes by Blue Native DIGE Katrin Peters and Hans-Peter Braun 11. 2D DIGE Analysis of Protein Extracts from Muscle Tissue Cecilia Gelfi and Sara De Palma 12. Combination of Highly Efficient Hexapeptide Ligand Library-Based Sample Preparation with 2D DIGE for the Analysis of the Hidden Human Serum/Plasma Proteome Sonja Hartwig and Stefan Lehr 13. 2D DIGE Analysis of Serum after Fractionation by ProteoMinerTM Beads Cynthia Liang, Gek San Tan, and Maxey C. M. Chung 14. Study Design in DIGE-Based Biomarker Discovery Alexandra Graf and Rudolf Oehler 15. Comparative 2D DIGE Analysis of the Depleted Serum Proteome for Biomarker Discovery Megan Penno, Matthias Ernst, and Peter Hoffman Part III. Applications in Clinical Proteomics 16. Differential Gel-Based Proteomic Approach for Cancer Biomarker Discovery Using Human Plasma Keun Na, Min-Jung Lee, Hye-Jin Jeong, Hoguen Kim, and Young-Ki Paik 17. 2D DIGE for the Analysis of RAMOS Cells Sub-Proteomes Marisol Fernández and Juan Pablo Albar 18. Application of Saturation Labeling in Lung Cancer Proteomics Gereon Poschmann, Barbara Sitek, Bence Sipos, and Kai Stühler 19. Proteomic Profiling of the Epithelial-Mesenchymal Transition Using 2D DIGE Rommel A. Mathias, Hong Ji, and Richard J. Simpson 20. Method for Protein Subfractionation of Cardiovascular Tissues before DIGE Analysis Athanasios Didangelos, Xiaoke Yin, and Manuel Mayr 21. Application of DIGE and Mass Spectrometry in the Study of Type 2 Diabetes Mellitus (T2DM) Mouse Models Celia Smith, Davinia Mills, and Rainer Cramer 22. Evaluating the Efficacy of Subcellular Fractionation of Blast Cells Using Live Cell Labeling and 2D DIGE Yin Ying Ho, Megan Penno, Michelle Perugini, Ian Lewis, and Peter Hoffman Part IV. Applications in Animal, Plant and Microbial Proteomics 23. DIGE Analysis of Plant Tissue Proteomes Using a Phenolic Protein Extraction Method Christina Rode, Traud Winkelmann, Hans-Peter Braun
- Kurztext
- This book introduces DIGE and its advantages in quantitative protein analysis, providing detailed protocols and important notes on the practical aspects of DIGE with both generic and specific applications in the various areas of Quantitative Proteomics.