Detailansicht
Protein Expression in Mammalian Cells
Methods and Protocols, Springer Protocols, Methods in Molecular Biology 801
ISBN/EAN: 9781617793516
Umbreit-Nr.: 1326730
Sprache:
Englisch
Umfang: xi, 276 S.
Format in cm: 2 x 26 x 18.5
Einband:
gebundenes Buch
Erschienen am 12.10.2011
Auflage: 1/2011
- Zusatztext
- Through all of the recent progress provided by high throughput DNA sequencing technologies, it has become clearer and clearer that the study of proteins and protein organelles will be the key to unlocking our ability to manipulate cells and intervene in human disease. In Protein Expression in Mammalian Cells: Methods and Protocols, expert researchers in the field present a compendium of vital techniques to further our knowledge of mammalian protein expression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. Authoritative and concise, Protein Expression in Mammalian Cells: Methods and Protocols will aid scientists seeking to delve deeper into our own biology through the medium of other mammalian cells and proteins.
- Kurztext
- Explores techniques to express proteins in mammalian cells in order to relate the research most closely to human biologyFeatures step-by-step methods and protocols, ready for the labIncludes expert tips and implementation advice to ensure successful results
- Leseprobe
- Inhaltsangabe1. Why Proteins in Mammalian Cells? James L. Hartley 2. Large Scale Transfection of Mammalian Cells Lucia Baldi, David L. Hacker, Carine Meerschman, and Florian M. Wurm 3. Selection of High Expressing Mammalian Cells by Surface Display of Reporters Christine T. DeMaria 4. Expression of a Secreted Protein in Mammalian Cells Using Baculovirus Particles Barbara Ann Jardin, Cynthia B. Elias, and Satya Prakash 5. Transfection of Difficult-to-Transfect Primary Mammalian Cells Oliver Gresch and Ludger Altrogge 6. Stable Protein Expression in Mammalian Cells Using Baculoviruses Andreas Lackner, Emanuel Kreidl, Barbara Peter-Vörösmarty, Sabine Spiegl-Kreinecker, Walter Berger, and Michael Grusch 7. Using Matrix Attachment Regions to Improve Recombinant Protein Production Niamh Harraghy, Montserrat Buceta, Alexandre Regamey, Pierre-Alain Girod, and Nicolas Mermod 8. Controlling Apoptosis to Optimize Yields of Proteins from Mammalian Cells Matthew P. Zustiak, Haimanti Dorai, Michael J. Betenbaugh, and Tina M. Sauerwald 9. Post-Transcriptional Regulatory Elements for Enhancing Transient Gene Expression Levels in Mammalian Cells Mariati, Steven C.L. Ho, Miranda G.S. Yap, and Yuansheng Yang 10. Converting Monoclonal Antibodies into Fab Fragments for Transient Expression in Mammalian Cells Joanne E. Nettleship, Aleksandra Flanagan, Nahid Rahman-Huq, Rebecca Hamer, and Raymond J. Owens 11. Generation of High-Expressing Cells by Methotrexate Amplification of Destabilized Dihydrofolate Reductase Selection Marker Say Kong Ng 12. Tools for Co-Producing Multiple Proteins in Mammalian Cells Zahra Assur, Wayne A. Hendrickson, and Filippo Mancia 13. Identification and Characterization of Protein Glycosylation Using Specific Endo- and Exoglycosidases Paula Magnelli, Alicia Bielik, and Ellen Guthrie 14. Strategies for the Efficient Transfection of CHO-Cells with Plasmid DNA Renate Kunert and Karola Vorauer-Uhl 15. Methods for Constructing Clones for Protein Expression in Mammalian Cells Takefumi Sone and Fumio Imamoto 16. Optimizing Transient Recombinant Protein Expression in Mammalian Cells Ralph F. Hopkins, Vanessa E. Wall, and Dominic Esposito