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Replacing Animal Models
A practical guide to creating and using biometric tissue alternatives
ISBN/EAN: 9780470974254
Umbreit-Nr.: 3208473
Sprache:
Englisch
Umfang: 214 S.
Format in cm:
Einband:
gebundenes Buch
Erschienen am 13.04.2012
Auflage: 1/2012
- Zusatztext
- Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. Replacing Animal Models provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments. The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for and potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing. The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so will result in both scientific and ethical gains.
- Kurztext
- Over the last decade, in vitro models have become more sophisticated and are at a stage where they can provide an effective alternative to in vivo experiments. Replacing Animal Models provides scientists and technicians with a practical, integrated guide to developing culture-based alternatives to in vivo experiments. The book is neither political nor polemical: it is technical, illustrating by example how alternatives can be developed and used and providing useful advice on developing others. After looking at the reasons for the potential benefits of alternatives to animal experiments, the book covers a range of methods and examples emphasising the design considerations that went into each system. The chapters also include 'case studies' that illustrate the ways in which culture models can be used to answer a range of important biological questions of direct relevance to human development, physiology, disease and healing. The thesis of this book is not that all animal experimentation can be replaced, now or in the near future, by equally effective or superior alternatives. Rather, the premise is that there is substantial opportunity, here and now, to do some common types of experiment better in vitro than in vivo, and that doing so well result in both scientific and ethical gains.
- Autorenportrait
- InhaltsangabeContributors, vii Preface, xi Section 1 Introductory Material 1 Potential Advantages of Using Biomimetic Alternatives, 3 Jamie Davies 2 Overview of Biomimetic Alternatives, 13 Jamie Davies Section 2 Culture Methods 3 Pancreatic Islets, 23 Eli C Lewis 4 Endometrial Organoid Culture, 35 Merja Blauer 5 Modelling Lymphatic and Blood Capillary Patterning, 45 Francoise Bruyere, Catherine Maillard, Charlotte Erpicum and Agnes Noel 6 Precisioncut Lung Slices (PCLS), 57 Christian Martin and Stefan Uhlig 7 Human Colon Tissue in Organ Culture, 69 Michael K Dame and James Varani 8 Fetal Organ Culture, 81 Jamie Davies 9 Design of a Mechanical Loading Device to Culture Intact Bovine Spinal Motion Segments under Multiaxial Motion, 89 Jochen Walser, Stephen John Ferguson and Benjamin Gantenbein-Ritter 10 Magnetic Assembly of Tissue Surrogates, 107 ChienYu Fu and HwanYou Chang 11 Assembly of Renal Tissues by Cellular Self-organization, 115 Mathieu Unbekandt Section 3 Case Studies of Use 12 Hierarchical Screening of Pathways: Using Cell and Organ Cultures to Reduce use of Transgenic Mice, 125 Guangping Tai and Jamie Davies 13 Lung Organoid Culture to Study Responses to Viruses, 137 Wenxin Wu, J Leland Booth and Jordan P Metcalf 14 Organcultured Human Skin for the Study of Epithelial Cell Invasion of Stroma, 151 James Varani 15 Organotypic Mandibular Cultures for the Study of Inflammatory Bone Pathology, 159 Alastair J Sloan, Sarah Y Taylor and Emma L Smith 16 Threedimensional, Highdensity and Tissue Engineered Culture Models of Articular Cartilage, 167 Ali Mobasheri, Sara Kelly, Abigail L. Clutterbuck, Constanze Buhrmann and Mehdi Shakibaei 17 Concluding Remarks, 193 Jamie Davies Appendix 1 Sources of funding for development of culture-based alternatives, 195 Appendix 2 Databases and web-based discussions relevant to development of alternatives, 197 Index, 199