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DNA Nanotechnology
Methods and Protocols, Methods in Molecular Biology 749
ISBN/EAN: 9781617791413
Umbreit-Nr.: 1536556
Sprache:
Englisch
Umfang: xii, 361 S., 92 s/w Illustr., 361 p. 92 illus.
Format in cm:
Einband:
gebundenes Buch
Erschienen am 27.06.2011
Auflage: 1/2011
- Zusatztext
- Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field.Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the experts
- Kurztext
- Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field.
- Autorenportrait
- Inhaltsangabe1. Synthesis and Characterization of Self-assembled DNA Nanostructures Chenxiang Lin, Yonggang Ke, Rahul Chhabra, Jaswinder Sharma, Yan Liu, and Hao Yan 2. Protocols for Self-assembly and Imaging of DNA Nanostructures Thomas L. Sobey and Friedrich C. Simmel 3. Self-Assembly of Metal-DNA Triangles and DNA Nanotubes with Synthetic Junctions Hua Yang, Pik Kwan Lo, Christopher K. McLaughlin, Graham D. Hamblin, Faisal A. Aldaye, and Hanadi F. Sleiman 4. DNA-Templated Pd Conductive Metallic Nanowires Khoa Nguyen, Stephane Campidelli, and Arianna Filoramo 5. A Method to Map Spatiotemporal pH Changes Inside Living Cells using a pH Triggered DNA Nanoswitch Souvik Modi and Yamuna Krishnan 6. Control of Helical Handedness in DNA and PNA Nanostructures Roberto Corradini, Tullia Tedeschi, Stefano Sforza, Mark M. Green, and Rosangela Marchelli 7. G-quartet, G-quadruplex, and G-wire Regulated by Chemical Stimuli Daisuke Miyoshi and Naoki Sugimoto 8. Preparation and Atomic Force Microscopy of Quadruplex DNA James Vesenka 9. Synthesis of Long DNA-based Nanowires Alexander Kotlyar 10. G-wire Synthesis and Modification with Gold-nanoparticle Christian Leiterer, Andrea Csaki, and Wolfgang Fritzsche 11. Preparation of DNA Nanostructures with Repetitive Binding Motifs by Rolling Circle Amplification Edda Reiß, Ralph Hölzel, and Frank F. Bier 12. Controlled Confinement of DNA at the Nanoscale: Nanofabrication and Surface Bio-functionalization Matteo Palma, Justin Abramson, Alon A. Gorodetsky, Colin Nuckolls, Michael P. Sheetz, Shalom J. Wind, and James Hone 13. Templated Assembly of DNA Origami Gold Nanoparticle Arrays on Lithographically Patterned Surfaces Albert M. Hung and Jennifer N. Cha 14. DNA-modified Single Crystal and Nanoporous Silicon Andrew Houlton, Bernard A. Connolly, Andrew R. Pike, and Benjamin R. Horrocks 15. The Atomic Force Microscopy as a Lithographic Tool: Nanografting of DNA Nanostructures for Biosensing Applications Matteo Castronovo and Denis Scaini 16. Trapping and Immobilization of DNA Molecules Between Nanoelectrodes Anton Kuzyk, J. Jussi Toppari, and Päivi Törmä 17. DNA Contour Length Measurements as a Tool for the Structural Analysis of DNA and Nucleoprotein Complexes Claudio Rivetti 18. DNA Molecular Handles for Single Molecule Protein Folding Studies by Optical Tweezers Ciro Cecconi, Elizabeth A. Shank, Susan Marqusee, and Carlos Bustamante 19. Optimal Practices for Surface-tethered Single Molecule Total Internal Reflection Fluorescence Resonance Energy Transfer Analysis Matt V. Fagerburg and Sanford H. Leuba 20. Engineering Mononucleosomes for Single-Pair FRET Experiments Wiepke J.A. Koopmans, Ruth Buning, and John van Noort 21. Measuring DNA-Protein Binding Affinity on a Single Molecule Using Optical Tweezers Micah J. McCauley and Mark C. Williams 22. Modeling Nanopores for Sequencing DNA Jeffrey R. Comer, David B. Wells, and Al